The tomato FISH map is a cytological map being generated as part of the Tomato Genome Sequencing project. The aim is to verify the genetic and physical maps, to explore the extent of the euchromatin, and to more precisely locate the euchromatin/heterochromatin boundary on tomato chromosomes. The results on this map are the work of Stephen Stack's lab at the Colorado State University, USA, but similar experiments are being conducted at Hans de Jong's lab at the University of Wageningen, the Netherlands, Zhukuan Cheng's lab at the Academy of Sciences in Beijing, China. Fluorescence In-Situ Hybridization (FISH) is performed on the pachytene phase chromosomes using labeled BAC probes. Some of the BAC probes have been linked to the genetic map through the overgo process (see physical map). The FISH map is displayed on pachytene glyphs, developed by Prof Stephen Stack, that represent the chromosomes in the pachytene phase of meiosis. The lighter color represents the euchromatin and the darker, thicker areas represent the heterochromatin. The location of the centromere is represented by a dark dot. The mapped BACs appear as red marks on the chromosomes.

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